Table 1.
Basic parameters of datasets used to test Gctf in this paper.
| Dataset ID | Sample | Type | Microscope | Detectors |
|---|---|---|---|---|
| 1 | Empty carbon | Raw grid | Spirit | Orius CCD |
| 2 | Dynein | Negative stain | Spirit | Orius CCD |
| 3 | Dynactin | Negative stain | Spirit | Orius CCD |
| 4 | Dynactin | On thin carbon | Krios | FalconII |
| 5 | Dynactin | On thin carbon | Krios | FalconII |
| 6 | Dynactin | On thin carbon | Krios | FalconII |
| 7 | Dynactin | On thin carbon | Polara | FalconIII |
| 8 | HAV | In pure ice | Polara | K2 summit |
| 9 | Chaperoin | In pure ice | Krios | UltraScan CCD |
| ID | apix | kV | Cs | Ac | Dose | Frames | Image size |
|---|---|---|---|---|---|---|---|
| 1 | 3.3 | 120 | 2.0 | 0.3 | 20 | 1 | 2048 × 2048 |
| 2 | 2.5 | 120 | 2.0 | 0.3 | 20 | 1 | 2048 × 2048 |
| 3 | 3.8 | 120 | 2.0 | 0.3 | 10 | 1 | 2048 × 2048 |
| 4 | 1.70 | 300 | 2.7 | 0.1 | 51 | 51 | 4096 × 4096 |
| 5 | 1.34 | 300 | 2.7 | 0.1 | 54 | 34 | 4096 × 4096 |
| 6 | 1.07 | 300 | 2.7 | 0.1 | 80 | 34 | 4096 × 4096 |
| 7 | 1.38 | 300 | 2.2 | 0.1 | 54 | 34 | 4096 × 4096 |
| 8 | 1.35 | 300 | 2.0 | 0.1 | 20 | 20 | 3712 × 3712 |
| 9 | 0.93 | 300 | 2.7 | 0.04 | 20 | 20 | 4096 × 4096 |
apix: Pixel size in Ångstrom.
kV: high tension of the micrographs.
Cs: spherical aberration coefficient.
Ac: amplitude contrast used for CTF determination.
Dose: Total dose of all frames, e/Å2.