Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Neurol. 2014 Dec 24;265:69–83. doi: 10.1016/j.expneurol.2014.12.012

FIGURE 1. L-type VOCC activation stimulates OPC maturation.

FIGURE 1

(A) One day after plating, OPCs were treated with high K+ and the L-type VOCC inhibitor verapamil. Verapamil (5µM) was added to the culture media for three consecutive days and high K+ (20mM) was applied in five consecutive pulses (5min/each) distributed during the first 5h of the second day in vitro. (B–C) After treatment OPCs were stained with antibodies against Olig1, NG2, MBP, MOG and CC1 and the percentage of positive cells in each experimental condition was examined by confocal microscopy. Scale bar = 60µm (Olig1, MOG, CC1); 80µm (NG2, MBP). (D) Morphological complexity of MBP-positive cells was scored in four categories. Values are expressed as mean ± SEM of four independent experiments. *p<0.05, **p<0.01, ***p<0.001 vs. respective controls.