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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Exp Neurol. 2014 Dec 24;265:69–83. doi: 10.1016/j.expneurol.2014.12.012

FIGURE 7. OPC viability after L-type VOCC knockdown.

FIGURE 7

OPCs were transfected with siRNA duplexes specific for Cav1.2 and Cav1.3 (siCav1.2/1.3) and grown as described in Figure 2. (A) Real-time Caspase-3 assay, using NucView 488 Caspase-3 substrate, was performed as described in Materials and Methods. Fluorescent field images were obtained with a specific GFP filter at 6min intervals for a period of 24h beginning 48h after siRNA transfection. Bright field images were superimposed to show the cell morphology. Yellow arrowheads designate some apoptotic OPCs (Caspase-3+ cells). Time is denoted in hours in the bottom right corner. Scale bar = 60µm. (B) OPCs death were evaluated by measuring the percentage of Caspase-3+ cells in each experimental group for a period of 24h. (C) Evaluation of OPCs viability by the MTT assay 48h after siRNA transfection. Values are expressed as mean ± SEM of five independent experiments.