Table 2.

Primers and thermocycling conditions

Primer	Sequence 5′ to 3′	Length of amplicon	Amplification conditions	Reference
amoA1F	GGGGTTTCTACTGGTGGT	490	5 min at 95°C, followed by    35 cycles of 45 s at 94°C,    45 s at 55°C, 1min at    72°C, with a final    extension at 72°C for 10    minutes	Rotthauwe et al. (1997)
amoA2R	CCTCKGSAAAGCCTTCTTC			Rotthauwe et al. (1997)
amoA-    1F-GC	CGCCCGCCGCGCCCCGC    GCCCGGCCCGCCGCCC    CCGCCCCGGGGTTTC    TACTGGTGGT			Shen et al.(2008)
FGPH19	TACGGCAARGGTGGNATHG	432	2 min at 94°C, followed by    30 cycles of 1 min at    94°C, 1 min at 57°C,    2 min at 72°C, final    extension of 5 min at    72°C	Simonet et al. (1991)
PolR	ATSGCCATCATYTCRCCGGA			Poly et al. (2001)
PolF AQER PolF GC	TGCGAYCCSARGCBGACTC GACGATGTAGATYTCCTG CGCCCGCCGCGCGCGGCGGGC    GGGGCGGGGGCACG    GGGGGTGCGAYCCSAR    GCBGACTC	321	2 min at 94°C, followed by    30 cycles of 1 min at    94°C, 1 min at 57°C,    2 min at 72°C, final extension of 5 min at 72°C	Poly et al. (2001) Poly et al. (2001) Demba-Diallo et al. (2004)