Figure 1.

Intracellular stability was monitored for X-LF_N-DTA constructs delivered through protective antigen pore. (a) ^LX- (left) and ^DX- (right) amino acids ligated to the N-terminus of LF_N-DTA (LF_N, green, pdb is 1J7N; DTA, orange, pdb is 1DTP). (b) XALPSTGG peptides, where X represents either an l- or d-amino acid, are ligated onto G₅-LF_N-DTA using sortase A (SrtA) to form X-LF_N-DTA constructs. (c) Translocation of X-LF_N-DTA constructs is achieved using protective antigen (PA) of anthrax toxin.