Fig. 5.

Effect of folate deficient and repletion diets on the expression of liver folate metabolite genes, determined by qRT-PCR. Relative transcript abundances of each gene were normalised to the housekeeping gene GAPDH. Values are means ± S.D. Group 1: Depleted (7 weeks), Group 2: Control (non-depleted, 9 weeks), Group 3: Depleted + 1.0 mg folate/kg diet from M. oleifera leaves (MO), Group 4: Depleted + 2.0 mg folate/kg diet from MO, Group 5: Depleted + 2.0 mg folate/kg diet from MO + ascorbic acid (AA), Group 6: Depleted + 2.0 mg folate/kg diet from folic acid (FA), Group 7: Depleted + 2.0 mg folate/kg diet from FA + AA. *Significant (P < 0.05) compared to non-depleted control (Group 2)