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(a) Comparison of the mathematically expected and measured VEGF-A levels after a 1:5 and 1:10 sample dilution. Both ELISA and multiplex bead array measurements measure slightly higher values than mathematically expected after sample dilution; (b) Addition of up to 1.333 pg/ml of recombinant VEGF-A to a plasma sample yields recovery rates of about 80% in all anticoagulants. Exp. = expected value; Meas. = measured value.
