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. 2014 Mar 27;1(2):024701. doi: 10.1063/1.4869472

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© 2014 Author(s).

2329-7778/2014/1(2)/024701/14

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FIG. 3. — Geometry to determined time-resolved structures from solution. (a) Optimal setup: serial single molecule diffraction. Jet diameter is small. Due to jet and X-ray beam instabilities the hit rate is very small, if not non-existent. (b) More realistic scenario: Jet diameter is larger. The coherent XFEL beam interacts with an ensemble. (c) Geometry already in reach: X-ray beam is focused to 10 nm to increase the photon area density. Jet is large to allow for instabilities. With a PYP concentration of 40 mg/ml, the number of molecules in the intersecting volume V would be ∼40.