Table 2. Enzymes for screening.
For comparison of enzyme activity equal protein concentrations (mg/mL) were used. The concentrations of all hydrolase solutions were determined by Bradford assay. Stock solutions of hydrolases were 10 mg/mL. All enzymes but pNB-Est13 were were purchased comerially. pNB-Est13 was hetrologous expressed in E. coli.
| Abbreviation | Hydrolase type | Origin | Molecular weigth |
|---|---|---|---|
| PPL | Lipase | Porcine pancreas | 50 kDa [61] |
| TLL | Lipase | Thermomyces lanuginosus | 30 kDa [62] |
| RML | Lipase | Rhizomucor miehei | 29 kDa [63] |
| CRL | Lipase | Candida rugosa | 60 kDa [64] |
| ALBC | Amano lipase PS | Burkholderia cepacia | 28 kDa [65] |
| ALPF | Amano lipase | Pseudomonas fluorescens | 32 kDa [66] |
| ALM | Amano lipase M | Mucor javanicus | 21 kDa [67] |
| pNB-Est13* | Esterase M | Bacillus licheniformis | 55 kDa [68] |
| HRP | Peroxidase | Armoracia rusticana | |
| AOX | Alcohol oxidase | Pichia pastoris |