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. 2015 Jan 12;13(1):73–81. doi: 10.1038/cmi.2014.126

Figure 4.

Figure 4

Tim-3 signaling inhibits inflammatory cytokine production by LPS-induced dNK cells. Purified dNK cells alone or cocultured with trophoblasts were treated with control or LPS for 48 h. Some cells were pretreated with rhGalectin-9 or anti-Tim-3 neutralizing Abs. The percentages of cells producing the inflammatory cytokine TNF-α (a), cytotoxic perforin (b) and IL-4 (c) in gated CD3−CD56+ dNK cells were determined using FCM after pre-stimulation with PMA, ionomycin and BFA for 4 h. The results are representative of nine independent experiments. Data are shown as the mean±s.e.m. **P<0.01, compared with dNK cells cultured alone; # P<0.05, ## P<0.01, ### P<0.001 compared with dNK cells treated with LPS; ▴▴ P<0.01, ▴▴▴ P<0.001 compared with dNK cells treated with LPS and rhGal-9; ▵▵▵ P<0.001 compared with dNK cells cocultured with trophoblasts and treated with LPS; dNK, decidual NK cells; FCM, flow cytometry; LPS, Lipopolysaccharide; Tro, trophoblast; α-Tim-3, anti-Tim-3 neutralizing antibodies.