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. 2015 Jan 12;13(1):73–81. doi: 10.1038/cmi.2014.126

Figure 5.

Figure 5

Decreased numbers of Tim-3+ dNK cells is accompanied by dysregulated Th2 predominance and higher cytotoxicity in human miscarriages. (a) FCM was used to analyze the relative number of Tim-3+ dNK cells in normal pregnancies and miscarriages. (b–f) dNK cells from normal pregnancies and miscarriages were treated with PMA, ionomycin and BFA for 4 h, and intracellular cytokines and perforin were detected using FCM. Histograms show Th2 cytokine (IL-4 and IL-10) (b, c), Th1 cytokine (TNF-α and IFN-γ) (d, e) and perforin (f) production by Tim-3+ and Tim-3− dNK cells from normal pregnancies and miscarriages. (g) Tim-3 expression on NK cells from decidual tissues of normal pregnant mice and abortion-prone mice during the first trimester. (h) FCM analysis of cytokine production by NK cells from decidua of normal pregnant mice and abortion-prone mice. Data are shown as the mean±s.e.m. of 10 different samples. *P<0.05; **P<0.01; ***P<0.001. NP, normal pregnancy. CBA/J×Balb/c, normal pregnancy mouse model; CBA/J×DBA/2, abortion-prone mouse model; dNK, decidual NK cells; FCM, flow cytometry.