Table 3. Allopurinol susceptibilities of intracellular amastigote strains.
Outcomes are presented as percent inhibition in cultures treated with 300μg/mL allopurinol relative to untreated controls.
| Isolate§ | Average amastigote count per 100 macrophages | Percent inhibition¶ | |
|---|---|---|---|
| Treated | Untreated | ||
| NT1 | 47.5±7.8 | 57.5±13.4 | 12.3±0.2 |
| NT2 | 98±0 | 119.5±6.4 | 17.9±4.4 |
| NT3 | 60±0 | 76±5.7 | 20.8±5.9 |
| NT4 | 68.5±0.7 | 91.5±4.9 | 25±3.3 |
| NT5 | 86.5±7.8 | 109±14.1 | 20.4±3.2 |
| NT6 | 68.5±4.9 | 89±9.9 | 22.9±3 |
| NT7 | 76.5±13.4 | 92.5±16.3 | 17.3±0 |
| NT8 | 66.5±4.9 | 76.5±4.9 | 13.1±0.8 |
| NT9 | 95±8.5 | 123±8.5 | 22.8±1.6 |
| NT10 | 99±9.9 | 136.5±6.4 | 27.6±3.9 |
| Mean ± STDEV | 20.0±4.9* | ||
| TR1 | 82±29.7 | 82.5±30.4 | 0.5±0.3 |
| TR2 | 75±1.4 | 79.5±3.5 | 5.6±2.4 |
| TR3 | 87±5.7 | 93.5±4.9 | 7±1.1 |
| TR4 | 58.5±4.9 | 66.5±4.9 | 12.1±0.9 |
| Mean ± STDEV | 6.3±4.8 | ||
§ Parasites isolated from NT (non-treated) or TR (treated relapsed) dogs.
¶ Percent Inhibition = [1- (Number of amastigotes in allopurinol treated DH-82 cells / Number of amastigotes in control untreated DH-82 cells)] x 100.
* Represents significant difference between groups (Mann-Whitney test, P = 0.002)