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. 2016 Jan 7;11(1):e0145986. doi: 10.1371/journal.pone.0145986

Fig 7. κFGF-MTS-anti-p24 mAb reduces HIV-1 infection of PBMCs.

Fig 7

(A) PHA -activated PBMCs were incubated with 10 μg/mL of κFGF-MTS-anti-p24 mAbs, native anti-p24 mAb, or unrelated κFGF-MTS-anti-AchE mAbs at 37°C for 18 h. Cells were washed and challenged with HIV-1 NL4.3 at MOI 0.1 for 2 h. Cells were co-cultured with 10 μg/mL of antibodies for up to 8 days and fed with fresh medium with antibodies every 48 h. Spent medium was harvested for MAGI assay. At each harvest, the cells were counted and re-plated to normalize the viral production at per cell base. The virus replication was quantitatively determined by counting the blue foci and plotted. (B) Activated PBMCs were infected with HIV-1 for 2 h. After 24 h, cells were treated with antibodies for up to 8 days. Cells were replenished with fresh medium containing antibodies every 48 h whereas spent medium was harvested for MAGI assay. The results are representative of three independent experiments each done in duplicate.