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Undifferentiated N2a cells were transfected with plasmids encoding for the closed (C) or open (O) variants of the LOV2-PAH1b or LOV2-RILPN313 chimeras, as indicated. The endogenous mRNA levels of the reported genes were quantified by qRT-PCR. GAPDH and HPRT1 were used as control housekeeping genes (one-way ANOVA followed by the Tukey’s multiple comparison test; n = 3 independent experiments).
