Table S1.
NanoString nCounter code set design
| Gene name | Accession no. | REST-target | Position | Target sequence |
| REST-target genes | ||||
| Stat1 | NM_009283.3 | YES | 1591–1690 | ACGCTGGGAACAGAACTAATGAGGGGCCTCTCATTGTCACCGAAGAACTTCACTCTCTTAGCTTTGAAACCCAGTTGTGCCAGCCAGGCTTGGTGATTGA |
| Nfasc | NM_001160316.1 | YES | 4936–5035 | CTCTTCGTATGCCTTATACAGCTCGGATCTAACCCTGCGGTTCCCAGATCCCTAGCCCTATTCTGCAAGTCTTGATGCCTCCAGTGATGTGTGTCTCGGG |
| Nrxn1 | NM_177284.2 | YES | 721–820 | AGATGCCCTCACTTAAACATCATCGGATGCAAATGGATCAGTGATCGCTCTTGAGCCTCGGTGGCCCTCTTTTTCAGAACGTTGCCTCCAAAGTGTATCC |
| Grin2b | NM_008171.3 | YES | 6341–6440 | GGGAAAGCTCTTCGTATAAGGCTTTGTGAAAGAGCCATTACAGTAGGGTGAGAGAGGGGGATGTTTTTAGTCATTAACGGTAGGGTTAGTGAGAAAGGGG |
| Cacna1h | NM_021415.4 | YES | 3213–3312 | ATCGTCACTGTGTTTCAGATCTTGACACAGGAAGACTGGAATGTGGTTCTTTACAACGGCATGGCTTCCACCTCGTCCTGGGCTGCCCTTTACTTTGTGG |
| Syp | NM_009305.2 | YES | 733–832 | TGTTTGGCTTCCTGAACCTGGTGCTCTGGGTTGGCAACCTATGGTTCGTGTTCAAGGAGACAGGCTGGGCCGCCCCATTCATGCGCGCACCTCCAGGCGC |
| Grin1 | NM_008169.2 | YES | 493–592 | ACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGTCACCCGCCTACTCCCAACGACCACTTCACTCCCACC |
| Cacna1i | NM_001044308.2 | YES | 9301–9400 | TGCCCTGGCTTATTCCTGTCTTCGGCTCTGGTCCTGGCTTTCCCTCAGAGGAGGATGAACGAATCATGAGCAGTACTTACCTGTCTGCTTGTCCCTAATA |
| Calb1 | NM_009788.4 | YES | 344–443 | ATGGAAAAATAGGAATTGTAGAGTTGGCTCACGTCTTACCCACAGAAGAGAATTTCTTGCTGCTCTTTCGATGCCAGCAACTGAAGTCCTGCGAGGAATT |
| Aplp1 | NM_007467.3 | YES | 2301–2400 | CTGGGTCCCAGGTATGTATGTCACTCCCTGGAATTCACCATCCCACGTTTCTTCACTAACATCCCAATAAAGTCCTCTTTCCCACCCGGCCAAAAAAAAA |
| Syt4 | NM_009308.3 | YES | 1121–1220 | TCTGGTCTCTCTCTGTTATCAGTCCACTACAAACACGCTCACTGTGGTGGTCTTAAAAGCGCGGCACCTACCGAAATCTGATGTGTCTGGACTTTCAGAT |
| L1cam | NM_008478.3 | YES | 3561–3660 | TGAAGACTAATGGAACTGGCCCTGTGCGAGTTTCTACTACAGGTAGCTTTGCCTCCGAGGGCTGGTTCATCGCCTTTGTCAGCGCTATCATTCTCTTGCT |
| Sst | NM_009215.1 | YES | 47–146 | CCTGCGACTAGACTGACCCACCGCGCTCCAGCTTGGCTGCCTGAGGCAAGGAAGATGCTGTCCTGCCGTCTCCAGTGCGCCCTGGCTGCGCTCTGCATCG |
| Neurod1 | NM_010894.2 | YES | 565–664 | CCTGCGCTCAGGCAAAAGCCCTGATCTGGTCTCCTTCGTACAGACGCTCTGCAAAGGTTTGTCCCAGCCCACTACCAATTTGGTCGCCGGCTGCCTGCAG |
| Bdnf | NM_007540.4 | YES | 3261–3360 | AGTCCCGTCTGTACTTTACCCTTTGGGGTTAGAAGTCAAGTTGGAAGCCTGAATGAATGGACCCAATGAGAACTAGTGTTAAGCCCATTTCCCTAGTCAG |
| Penk | NM_001002927.2 | YES | 1011–1110 | CGCTTTGCTGAGTCTCTGCCCTCCGATGAAGAAGGCGAAAATTACTCGAAAGAAGTTCCTGAGATAGAGAAAAGATACGGGGGCTTTATGCGGTTCTGAA |
| Glra3 | NM_080438.2 | YES | 757–856 | GGACTTGCGGTACTGCACTAAACACTACAATACAGGAAAGTTTACATGCATAGAAGTGCGATTCCATCTTGAGCGTCAGATGGGCTATTACTTGATCCAG |
| Gabrg2 | NM_177408.5 | YES | 1614–1713 | TTAGAACAAGAGCTGTTACACTGAGCAAGATACCTTTGAGCAACAGCAATGAAAACAGTGGAAGCTGGGAGGGTTTAAAGTGGCATTATCAGTCTTTGAC |
| Oprm1 | NM_001039652.1 | YES | 1197–1296 | GATTCCAGAAACCACTTTCCAGACTGTTTCCTGGCACTTCTGCATTGCCTTGGGTTACACAAACAGCTGCCTGAACCCAGTTCTTTATGCGTTCCTGGAT |
| Cacng2 | NM_007583.2 | YES | 645–744 | GACGCGGACTACGAAGCTGACACCGCAGAGTATTTCCTCCGGGCCGTGAGGGCCTCGAGTATCTTCCCGATCCTGAGTGTGATCCTGCTTTTCATGGGTG |
| Nrxn3 | NM_001198587.1 | YES | 6111–6210 | CTCAAAATCTACCATGGCATTCACTCCATGTAGCAGGTTGTGGTGTCTCTAGAACCAATTGTTAGTTTCCTAATGCTTTGGTGAACCCATGTGGGATGAC |
| Non–REST-target and housekeeping genes | ||||
| Map2k2 | NM_023138.4 | NO | 2268–2367 | CACATTCCTAAATACTAGGAAGGCTGAGTCGGGAAAACGACAGGTTTTGGGCCACTGTGGGCTACCTAGTGAGAATGTCTTACATCATGGAAATGGTGCA |
| Gtf3c1 | NM_207239.1 | NO | 4587–4686 | ACCAACGGCATGCTAGACCAGCCTGATCATTTTTCTTTCAAGGACCTGGATAGCAGTGACCCTTCAAATGACCTGGTGGCATTTTCTTTGGACAGCCCTG |
| Tuba1a | NM_011653.2 | NO | 1379–1478 | GGGAGGAAGAAGGAGAGGAATACTAAATTAAATGTCACAAGGTGCTGCTTCCACAGGGATGTTTATTGTGTTCCAACACAGAAAGTTGTGGTCTGATCAG |
| Camkk2 | NM_145358.2 | NO | 1321–1420 | AAAGGCGTTGGATGTTTGGGCCATGGGTGTGACGCTGTACTGCTTTGTCTTTGGCCAGTGCCCTTTCATGGATGAACGAATCATGTGTTTGCACAGTAAG |
| Syt17 | NM_138649.1 | NO | 1391–1490 | AACTGGAAAACGCCAGCCTAGTATTCACAGTGTTCGGCCACAACATGAAAAGCAGCAATGACTTCATCGGGAGGATCGTCATCGGCCAGTATTCCTCCGG |
| Ubc | NM_019639.4 | NO | 22–121 | GCGGAGTCGCCCGAGGTCACAGCCCTGCCCTCCCACACAAAGCCCCTCAATCTCTGGACGCCACCGTGAAACAACTCCGTGAGAGAGACGATGCAGATCT |
| Gpi1 | NM_008155.3 | NO | 2676–2775 | CAGGGATGAACTCGATTATACCCTTCAAAAGACAGTTTTAGCAGGGTGTCATTACTGTCACATTCATATCCTGGCTATCAGTGAGTCCTTGTGAGACTGA |
| Map3k5 | NM_008580.4 | NO | 641–740 | CATCATCCTCTACTGCGATACTAATTCCGATTCACTCCAGTCCCTGAAGGAAATTATTTGCCAGAAGAATACTGTGTGCACCGGGAACTACACCTTCATC |
| Rcan1 | NM_001081549.1 | NO | 1061–1160 | ACACAAGGACACTGGGGACATCCTGAGAAAACTGATAGTTCTTGTAATTGCTCATTTCTAGGTTCTGTTTTTGGCAAGGACAGGTTGACTGGTGGCCCAG |
| Tbx21 | NM_019507.1 | NO | 626–725 | CACTAAGCAAGGACGGCGAATGTTCCCATTCCTGTCCTTCACCGTGGCTGGGCTGGAGCCCACAAGCCATTACAGGATGTTTGTGGATGTGGTCTTGGTG |
| Tdo2 | NM_019911.2 | NO | 496–595 | AGAGTCTACAGTTCCGGCTGCTGGAAAATAAGATTGGTGTTCTTCAGAGCTTGAGAGTCCCTTACAACAGGAAACACTATCGTGATAACTTTGGAGGAGA |
| Gapdh | NM_001001303.1 | HK | 891–990 | AGGTTGTCTCCTGCGACTTCAACAGCAACTCCCACTCTTCCACCTTCGATGCCGGGGCTGGCATTGCTCTCAATGACAACTTTGTCAAGCTCATTTCCTG |
| Hdac3 | NM_010411.2 | HK | 1026–1125 | GGCCATTAGTGAGGAACTTCCCTATAGTGAATACTTCGAGTACTTTGCCCCAGATTTCACACTCCATCCAGATGTCAGCACCCGCATCGAGAATCAGAAC |
| Hprt | NM_013556.2 | HK | 31–130 | TGCTGAGGCGGCGAGGGAGAGCGTTGGGCTTACCTCACTGCTTTCCGGAGCGGTAGCACCTCCTCCGCCGGCTTCCTCCTCAGACCGCTTTTTGCCGCGA |
| Pgk1 | NM_008828.2 | HK | 37–136 | CCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTCACGGTGTTGCCAAAATGTCGCTT |
| Ppia | NM_008907.1 | HK | 391–490 | CCAAGACTGAATGGCTGGATGGCAAGCATGTGGTCTTTGGGAAGGTGAAAGAAGGCATGAACATTGTGGAAGCCATGGAGCGTTTTGGGTCCAGGAATGG |
Optimal sequences were designed on the chosen genes by Nanostring Technologies. The table reports the following for each gene: (i) the gene name; (ii) the accession number; (iii) whether it is a REST-target gene (YES/NO) or a housekeeping (HK) gene; the position (iv) and the sequence (v) of the targeted region.