Fig. 2.

Heterogeneity within fibroblast-like cells. (A) Subset analyses. First column (1–3), CD71 and CD54 subsets; subsequent columns, analysis of the CD71⁺ CD54⁻ subset by CD24, CD80, and CD90. Top row (1, 4, 5, 6), CD146⁺ CD73⁺ population; middle row (2, 7, 8, 9), CD146⁻ CD73⁺ population; bottom row (3, 10, 11, 12), CD146⁺ CD73⁻ population. (B) Stability of surface phenotype. Cultures initiated from subpopulations were reanalyzed after propagation in culture for four to six passages. Top row (1–3), reanalysis of CD54⁻ CD73⁻ Sca⁺ CD80⁻ cells that were originally CD24⁻ CD90⁻ (1), CD24⁻ CD90⁺ (2), or CD24⁺ CD90⁺ (3). Bottom row (4 and 5), CD54⁻ CD73⁻ Sca⁺ CD80⁺ cells, originally CD24⁻ CD90⁻ (4) or CD24⁻ CD90⁺ (5). (C) Heterogeneity of cell populations derived from isolated colonies. Individual colonies were picked and expanded, and the surface expression patterns of the progeny were analyzed by flow cytometry.