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. 2015 Dec 22;113(1):170–175. doi: 10.1073/pnas.1522297113

Fig. 3.

Fig. 3.

Chromatographic isolation and characterization of EVs from the medium. (A) Preparation and characterization of CD63+ EVs from medium of MSCs incubated as in Fig. 1G. (i) Assay by SDS-electrophoretic gel of medium eluted from anion exchange column with 0.5 M NaCl. Gel was silver-stained. (ii) Assays of eluted fractions for protein and CD63. (B) Recovery of CD63+ protein from the column was slightly greater than recovery by centrifuging the same samples at 100,000 × g for 12 h. (C) Assays of eluted fractions by nanoparticle diffusion analysis demonstrated that the mean size of the vesicles ranged from 209 ± 1.8 nm (SEM) to 231 ± 3.2 nm. The three peaks at the lower concentration (ii) were 85, 165, and 236 nm. (Insets) Photos of nanoparticles in the instrument.