Fig. 3.

CRY1 affects the high temperature-induced elevation of YUC8 transcripts and the free IAA level. (A and B) qPCR results showing high temperature-induced expression patterns of YUC8, IAA19, and IAA29 of the indicated genotypes. Four-day-old seedlings grown in 22 °C in continuous white light condition were transferred to 28 °C or were continually placed at 22 °C for a 5-h time course (A) or for only 3 h (B), respectively. (C) Hypocotyl length of 4-d-old WT, GFP-CRY1, and cry1 seedlings grown in the 22 °C or 28 °C continuous blue (40 μmol⋅m⁻²⋅sec⁻¹) conditions, with and without the synthetic auxin picloram. The hypocotyl lengths were measured and are shown in C. SDs (n > 15) are indicated. Hypocotyl length ratios (Picloram/Mock) of the quantified hypocotyl length in C are shown in D. (E) IAA concentrations of 6-d-old WT, cry1, and GFP-CRY1 seedlings grown in 20 °C and 28 °C continue white light (40 μmol⋅m⁻²⋅sec⁻¹) condition.