Fig. S4.

CRY1 represses the transcription of PIF4, but does not affect the protein stabilization of PIF4. (A) qPCR results showing that the transcription level of PIF4 is markedly increased in the cry1 mutant. Four-day-old seedlings grown in 22 °C continuous white light condition were transferred to 28 °C for a 5 h time course, respectively. Error bars represent SD of three technical repeats. Each experiment was performed at least three times with similar results. (B) Immunoblots showing that CRY1 did not affect the abundance of the PIF4 proteins in 4-d-old seedlings under the conditions tested. Samples were fractionated by 10% SDS/PAGE, blotted, and probed with the anti-MYC antibody. PIF4-TAP in WT or cry1 backgrounds were grown in 22 °C continuous blue light condition for 4 d, then transferred to 28 °C continuous blue light condition for the indicated time before sample collection.