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. 2015 Dec 22;113(1):224–229. doi: 10.1073/pnas.1511437113

Fig. S6.

Fig. S6.

CRY1 interacts with PIF3. (A) Phenotypic analysis. Seedlings of the indicated genotypes were grown in the 22 °C or 28 °C continuous white light (40 μmol⋅m–2⋅sec–1) for 4 d. The hypocotyl lengths of the indicated genotypes were measured and are shown. SDs (n > 15) are indicated. (B) In vitro pull-down assays showing interaction between CRY1 and PIF3. GST-tagged PIF3 or GST bound to GST beads was mixed with CRY1 purified from insect cells. The bound proteins were eluted after removing unbound proteins by washing, and analyzed by immunoblot probed with the anti-CRY1 (CRY1) or the anti-GST antibody (PIF3 and GST). (C) Co-IP assays of samples prepared from 6-d-old WT or 35S::PIF3-MYC seedlings grown in long-day condition (16 light/8 dark). Total proteins (Input) or IP product of anti-CRY1 antibody (CRY1-IP) were probed, in immunoblots, by the anti-CRY1 antibody (CRY1), stripped, and reprobed by the anti-MYC (PIF3-MYC) antibody.