Fig. 4.

Autophagy is neither a cytoprotective nor a cytotoxic response to anticancer treatments. (A) A549 ATG7 wild-type (clone 20) or ATG7-null (clone 3) cells were treated with increasing concentrations of various chemotherapeutic agents for 5 d. Cells were fixed and stained with SRB. The IC₅₀ value of each agent was calculated using GraphPad Prism. Each data point represents the IC₅₀ of the ATG7 wild-type line plotted against the IC₅₀ of the ATG7-null line. Individual IC₅₀ values and drug identities are displayed in Table S2. (B) A549 ATG7 wild-type (clone 20) or ATG7-null (clone 3) cells were treated with the indicated amounts of radiation. After 10 d, the number of surviving colonies was quantified.