Fig. S4.

Loss of ATG7 in HCT116 or PaTu-8988T cells does not impact proliferation. (A) HCT116 ATG7 wild-type and the ATG7-null clones 1E9 and 2A5 were probed by Western blot. (B) Cells were grown in 96-well plates, and confluence was measured at the indicated time points by the IncuCyte imaging system. (C) PaTu-8988T cells expressing DOX-inducible TALENs targeting ATG7 were pretreated with DOX as described in Materials and Methods and then were lysed and immunoblotted for autophagy pathway components. (D) Equal numbers of inducible ATG7-null cells treated as in C were plated, and proliferation assessed after 4 d by cell counting after Trypan Blue exclusion.