Fig. S5.

Inhibition of autophagy with dominant-negative ATG4B. (A) Panc 10.05 cells with DOX-inducible expression of ATG4B^C74A were cultured in the absence or presence of DOX for 5 d, and bafilomycin A1 was added for the last 24 h where indicated. Cells were lysed and probed by Western blot. (B) Panc 10.05 ATG4B^C74A cells were grown for the indicated times with or without DOX, and steady-state levels of ATG4B, p62, LC3, and GAPDH were visualized by Western blot. (C) Panc 10.05 ATG4B^C74A cells were cultured in 96-well plates with or without DOX, and cell growth was assessed using CellTiter-Glo at the indicated time points. Cell growth is depicted relative to day 0; data points represent the mean ± SD from two independent experiments.