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. 2016 Jan 13;12(1):e1005382. doi: 10.1371/journal.ppat.1005382

Fig 1. H. pylori-induced ROS generation is mediated by Rac1.

Fig 1

(A-C) ROS generation was measured by luminol oxidation in AGS cells (A), NCI-N87 cells (B) and primary gastric epithelial cells (C) after infection with H. pylori 26695 for 10, 30 or 60 min or left uninfected. (D) H. pylori-induced ROS generation (after 30 min) was measured in AGS cells after transfection with empty vector (pcDNA), constitutively active Rac1 (V12) or after treatment with Rac1 inhibitor (NSC23766). For A-C fold change of luminol oxidation compared to uninfected cells are shown as mean values (± SEM) of three independent experiments. (E-F) Rac1 activation was measured by a GTP pull down assay in AGS (E) and NCI-N87 (F) cells following infection with H. pylori at the indicated times. A representative western blot was selected from three independent experiments and densitometry results from multiple experiments are shown. Levels of significance are indicated as follows: * p<0.05, ** p<0.01 and *** p< 0.001.