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. 2016 Jan 13;12(1):e1005382. doi: 10.1371/journal.ppat.1005382

Fig 2. H. pylori-induced ROS generation is regulated by NADPH oxidase.

Fig 2

ROS generation was measured in (A) AGS cells infected with H. pylori for 30 min or left uninfected in the presence of 10 mM NAC or 10 μM DPI, (B) in Nox1 downregulated AGS cells following infection with H. pylori for 30 min or left uninfected and (C) in AGS cells either transfected with pcDNA or Rac1 V12 following infection with H. pylori for 30 min. In other experimental conditions, AGS cells were treated with NSC23766 (NSC), or DPI or both before infection with H. pylori for 30 min. For A-C fold change of luminol oxidation compared to uninfected cells are shown as mean values (± SEM) of three independent experiments. (D) Nox1 expression was measured in uninfected and H. pylori infected AGS cells transfected with either vector (pcDNA) or Rac1 V12 or were pre-treated with NSC23766 (NSC). The representative western blot with Nox1 expression was selected from three independent experiments and densitometry results are shown. Levels of significance are indicated as follows: ** p<0.01 and *** p< 0.001.