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. 2016 Jan 13;12(1):e1005382. doi: 10.1371/journal.ppat.1005382

Fig 6. Differential regulation of Rac1 activity and ROS generation by two functional domains of APE1.

Fig 6

(A) AGS cells were transfected with either vector (pFLAG-CMV-5.1), WT APE1, or K6R/K7R or H309N mutants and infected with H. pylori for 1h. The cell lysate was immunoprecipitated with anti-FLAG beads and analyzed for Rac1 and FLAG levels by western blot. Part of the cell lysate was used as an input to show the endogenous level of Rac1 and the corresponding APE1-FLAG expression. (B) The same set of transfected shRNA cells from (A) were infected with H. pylori for 30 min or left uninfected and ROS was measured by luminol oxidation. Mean values (± SEM) of five independent experiments are shown. (C) shRNA cells were transfected as in (A) and then infected with H. pylori for 1h and Rac1 activity was measured. A representative western blot was selected from three independent experiments. Levels of significance are indicated as follows: * p<0.05 and *** p< 0.001.