Fig 7. APE1 inhibits ROS generation in gut epithelial cells following infection with Salmonella enterica serovar Typhimurium (SL1344).

(A) HT-29 cells and (B) T84 cells were infected with Salmonella SL1344 or ΔSPI2 mutant for 30 min or left uninfected. Fold change of luminol oxidation compared to uninfected cells are shown as mean values (± SEM) of three independent experiments. (C) ROS generation detected by CM-H₂DCFDA was analyzed by confocal microscopy in WT or shAPE-transfected T84 cells after infection with SL1344 and ΔSPI2 for 30 min or left uninfected. DAPI was used for nuclear staining; scale bar indicates 100 μm. Levels of significance are indicated as follows: ** p<0.01 and *** p< 0.001.