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. 2015 Aug 12;93(4):83. doi: 10.1095/biolreprod.115.131193

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© 2015 by the Society for the Study of Reproduction, Inc.

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FIG. 5 — Expression of adult Leydig cell markers in Nr5a1^−/−;tg^+/0 mice. Semiquantitative RT-PCR was used to measure mRNA levels for the ALC-specific genes Sult1e1, Vcam1, and Hsd3b6 (Hsd3β6) and the control genes Cyp11a1 and Rpl7. Template was generated using total RNA isolated from wild-type (wt), Nr5a1^−/−;tg^+/0 cryptorchid (Rc), and Nr5a1^−/−;tg^+/0 descended (Rd) adult (2-mo-old) testes and Nr5a1^+/− P5 testes. A) Representative Southern blot of amplified signals for Sult1e1, Vcam1, Hsd3b6 (Hsd3β6), and Rpl7. B–D) Graphical representation of Rpl7 (black bars) and Cyp11a1 (gray bars) normalized Sult1e1 (B), Vcam1 (C), and Hsd3b6 (Hsd3β6) (D) signals from Rc, Rd, and P5 testes relative to those of wt testes. Autoradiographic signals were quantified by densitometry and values for ALC-specific mRNAs were normalized to values for either Rpl7 or Cyp11a1. Normalized values from Rd, Rc, and P5 testes were then made relative to the normalized values from wt testes.