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. 2015 Sep 9;93(4):89. doi: 10.1095/biolreprod.115.133504

FIG. 5.

FIG. 5

Combined Sin3a siRNA and morpholino inhibit maturation-associated increase in the amount of SIN3A. A) Full-grown oocytes microinjected with control or Sin3a morpholino (MO) and siRNA were cultured for 1 h in medium containing 2.5 μM milrinone and then cultured in inhibitor-free medium for maturation. MII eggs were collected 16 h after maturation and used for immunoblot analysis to detect SIN3A protein levels. TUBA was used to normalize total protein loading. The experiment was performed three times, and a representative example is shown. B) Quantification of the relative amount of SIN3A shown in A. The data are expressed as mean ± SEM.