FIG. 6.

Effect of inhibiting the maturation-associated increase in SIN3A on histone acetylation in 1-cell embryos. A) The indicated histone were assayed by immunocytochemistry for their acetylation status using maternal SIN3A-depleted, control 1-cell embryos (Ctrl), and 1-cell embryos that were incubated with TSA (an HDAC inhibitor) to generate the maximum increase in histone acetylation. The experiment was performed twice, and at least a total of 10 embryos were analyzed for each sample. Bar = 50 μm. B) Quantification of the data shown in A. The data are expressed relative to the control 1-cell embryos and are expressed as mean ± SEM. *P < 0.05.