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Inhibiting the maturation-associated increase in SIN3A leads to arrest at the 2-cell stage. After inhibiting the maturation-associated increase in SIN3A, the maternal SIN3A-depleted and control MII eggs were in vitro fertilized (IVF) and embryo development was assessed at 24, 48, and 72 h after fertilization; hpf, hours postfertilization. The experiment was performed three times and the data pooled. A total of 56 experimental and 43 control embryos were analyzed.
