FIG. 7.

Effects of in utero exposure to genistein (GEN) and DEHP on antioxidant protein and xenobiotic transporter gene expression. Immunohistochemical analysis of NQO1 was performed on PND3 testes (A) and positive staining was quantified by image analysis (B) (photos taken at 40× magnification). Immunohistochemical quantification is represented by mean fold change ± SEM of NQO1 staining relative to control (n ≥ 3). Omission of the primary antibody was used as a negative control (data not shown). Representative pictures are presented. Relative mRNA expression of xenobiotic transporters Abcb1b (C) and Abcg2 (D) in PND3 and PND6 testes. Data are expressed as mean relative mRNA levels ± SEM normalized to alpha-tubulin (n = 4 per treatment). Asterisks in both mRNA and protein analyses indicate a significant difference relative to control (P ≤ 0.05); C, control; G, GEN; D, DEHP; G + D, GEN + DEHP.