FIG. 2.

Changes in Prkaa1 (a) and Prkaa2 (b) gene and AMPK-α1 and AMPK-α2 protein (c) expression following 8 wk of exposure to cigarette smoke. Analysis of Prkaa1 and Prkaa2 gene and AMPK protein expression was performed on whole-ovarian homogenates from sham and smoke-exposed mice. a and b) qPCR gene expression changes relative to Actb used as a loading control (n = 6 per group). c) Representative immunoblot showing protein expression changes relative to the ACTB loading control (n = 7 sham and 6 smoke). Data were checked for normality and equal variance, and treatment effects were tested using t-test. Values are expressed as mean ± SEM. *P < 0.05.