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. Author manuscript; available in PMC: 2016 Jan 13.
Published in final edited form as: Cytoskeleton (Hoboken). 2014 Jun 26;71(6):380–394. doi: 10.1002/cm.21181

Figure 7. Expression of Arf GAPs inhibits ventral actin wave formation in Beas-2b cells.

Figure 7

(A) Flag-ASAP1, Flag-ASAP1 RK, Flag-ACAP1 or Flag-ACAP1 RQ was transfected into Beas-2b cells. Cells were treated with PMA and stained with anti-Flag antibody (left column) and rhodamine phalloidin to detect F-actin (middle column). ASAP1 and ACAP1 transfected cells largely did not exhibit ventral actin waves, while cells transfected with enzymatically inactive control constructs ASAP1 RK and ACAP1RQ retained ventral actin waves. Bar, 10μm. (B) Quantification of percentage of control or transfected cells exhibiting ventral actin waves after PMA treatment. A minimum of 100 cells in total was counted from at least 3 independent experiments. Bars represent standard error for proportional data. Percentage of cells transfected with ASAP1 or ACAP1 was significantly reduced compared to control cells (P<0.0001) as assessed by calculating P-values using Fisher’s Exact test (two-tailed.) Percentage of cells expressing ASAP1 RK or ACAP1RQ exhibiting ventral waves was not statistically different than control cells.