Fig 3. Effect of L-NAME, NS398 and Tempol on response to Ach at the clinical onset of arthritis.

(A) The vasodilator response to Ach (expressed as AUC of Ach) was assessed in aortic rings from AIA and controls at day 11 post-immunization. The same experiment was performed after incubation with L-NAME (B) (the insert presents AUC of Ach), NS398 (C) and Tempol (D). Results are expressed as means ± SEM of 12 to 18 aortic rings from 18 rats/group. *p<0.05, *** p<0.001.