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. 2016 Jan 13;12(1):e1005388. doi: 10.1371/journal.ppat.1005388

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© 2016 Graindorge et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Endogenously tagged myosin light chains (MLC) 3, 5 and 7 localized to the conoid. Scale bar 2 μm. (B) MLC5 localization at the conoid is dependent upon the presence of TgMyoH. Scale bar 2 μm. (C) MLC7 localization at the conoid is independent on the presence of TgMyoH. Scale bar 2 μm. (D) No decrease of the MLC5 level was observed by western blot upon depletion of TgMyoH. Profilin (PRF) serves as loading control. (E) A decrease of the MLC7 level was observed by western blot upon depletion of TgMyoH. Profilin (PRF) serves as loading control. (F) Plaque assays performed with RH (parental strain), mlc5-KO, mlc7-KO and mlc5&7-KO lines and fixed after 7 days showed no defect in the lytic cycle. (G) TgMyoH localization at the conoid is independent on the presence of MLC5 and MLC7. (H) Metabolic labeling followed by Co-IP experiments performed with anti-Ty antibodies on MyoH-3Ty and MyoH-3Ty-mlc5&7-KO strains. A band corresponding to the size of MLC1 was detected.