Fig 8. CFU-C assay of CD45+ engrafted cells recovered from bone marrow of NSG mice 12–15 week post transplantation with lentiviral transduced CD34+ cells.

(a)–(d) Average colony numbers from four different human donors transplanted without expansion after transduction process. Donor 4 samples were inadvertently plated at one-third the intended number of cells per plate. (e)-(g) Average colony numbers from three different human donors transplanted after expansion in liquid culture for one week. Donor 4 samples were inadvertently plated at one-third the intended number of cells per plate. Donor 1 did not have sufficient CD34+ cells to perform the expanded cell experiments. Statistical comparisons performed using two-tailed upaired t-test with a p-value of 0.05 being considered significant.