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. Author manuscript; available in PMC: 2016 Jan 13.
Published in final edited form as: Methods Mol Biol. 2015;1298:107–116. doi: 10.1007/978-1-4939-2569-8_9

Figure 2. Co-localization of interactions Ypt1 with its autophagy-specific GEF (Trs85) and effector (Atg11) using multi-color BiFC.

Figure 2

Yeast cells were transformed with three plasmids expressing: i) Y/CFP-C-Ypt1, Ypt1 was tagged at its N terminus with the C terminal fragment of Y/CFP; ii) YFP-N-Atg11, Atg11 tagged at its N terminus with the N terminal fragment of YFP; and iii) Trs85-CFP-N, Trs85 tagged at its C terminus with the N terminal fragment of CFP. Every cell shows multiple puncta in the CFP channel representing the Ypt1-Trs85 interaction. One of these puncta (arrows) co-localizes with the single punctum in the YFP channel representing the Ypt1-Atg11 interaction (merge). Size bar, 5 μm. See details in Lipatova et al, 2012 (4).