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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Nat Med. 2015 Oct 5;21(11):1280–1289. doi: 10.1038/nm.3949

Figure 1. Genome-wide miRNA screen identifies novel regulators of LDLR activity.

Figure 1

a, Schematic workflow of primary screen and bioinformatic procedures. b, Linear regression analysis between DiI-LDL mean average intensity (MAI) for plate set 1 and 2 (top), plate set 2 and 3 (middle) and plate set 1 and 3 (bottom). The goodness of fit (r2) and regression line (indicative of overall reproducibility of the screen) is indicated in red on each graph. c, DiI-LDL mean average intensity (MAI, open bars) and robust z-score (red dots) comparison for cells transfected with a negative control siRNA (non-silencing, NS) or positive control siRNA (siRNA LDLR, siLDLR). d, Distribution of average robust z-scores for individual miRNAs in the primary screen. Controls are represented by the grey (NS siRNA) and blue (siLDLR) dots. miR-148a, highlighted in green, was chosen for further validation based on predefined criteria (a). All other miRNAs are shown in black. Dashed red line, robust z-score = −1.5.