Cardiac fibrosis (A-D, F) was compared among five groups at 3 weeks after mesenchymal stem cells(MSCs) delivery(n = 4). Cardiac fibrosis was less in RIPoC+IPoC, RIPoC and IPoC group than that in IR group(p<0.01). Group A: RIPoC+IPoC group, received the combined algorithms of RIPoC and IPoC; Group B: RIPoC group, which received 3 cycles of 30 seconds reperfusion and re-occlusion on the limb at the onset of reperfusion; Group C: IPoC group, received 3 cycles of 30 seconds reperfusion and re-occlusion on the LAD; Group D: IR group, received reperfusion without interruption after ischemia; Group F: NAC+IR group, ROS scavenger N-acetylcyseine was administered in IR group.