Figure 4.

RNA-Seq Reveals Overlapping Pathways Targeted by Both Prmt1 and Kdm4c

(A) Heatmap analysis of gene expression profile in MOZ-TIF2 and MLL-GAS7 leukemic cells after Prmt1knockout and Kdm4c knockdown.

(B) Venn diagram showing the common downregulated pathways after the loss of function of Prmt1 and Kdm4c (overlapped) identified by GSEA.

(C) Heatmap analysis of gene expression signature perturbed in both MLL-AF9 and MLL-GAS7 after Kdm4c knockdown.

(D) Venn diagram showing the commonly downregulated genes on MLL-AF9 and MLL-GAS7 after Kdm4c knockdown (left) as well as commonly downregulated pathways identified by GSEA (right).

(E) Embryonic stem cell signature and Myc pathway were downregulated after the loss of Prmt1 or Kdm4c in both MOZ-TIF2 (left) and MLL-GAS7 (right).

(F) Embryonic stem cell signature and Myc pathway were downregulated in both MLL fusions after Kdm4c knockdown.

(G) qRT-PCR analysis of Myc and Bcat1 expression after Kdm4c knockdown.

(H) ChIP analysis showing the effect of Kdm4c knockdown on H3K9me3 mark in Myc loci of MLL-AF9 leukemia cells.

All data shown are mean and SD (n = 3) unless otherwise specified. See also Figure S4.