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. Author manuscript; available in PMC: 2016 Jan 13.
Published in final edited form as: J Cyst Fibros. 2013 Feb 9;12(6):773–779. doi: 10.1016/j.jcf.2013.01.001

Fig. 1.

Fig. 1

CFBE41o- and CFBE41o-/CEP-CFTR cells are hyporesponsive to M. abscessus and the TLR2/TLR1 ligand Pam3Cys as measured by HβD2 gene expression. CFBE41o- cell monolayers received no treatment or were treated with IL-1β, MALP-2 and the rough M. abscessus variant 390R (A), or the TLR2/TLR1 ligand Pam3Cys (100 ng/ml) (B). After 8 h, HβD2 gene expression was quantified by real-time PCR. The results of real-time PCR are expressed as the relative fold increase in HβD2 gene expression over that of the untreated group and presented as mean+/−SEM of measurements from duplicate experiments performed in triplicate. Asterisks note differences, p<0.05, t-test. In addition, CFBE41o-/CEP-CFTR cells received the same treatment, substituting increasing concentrations of Pam3Cys (100, 1000 and 10,000 ng concentrations) for Pam3Cys 100 ng/ml and M. abscessus 390R (C). Asterisks denote differences, p<0.05, t-test.