Fig. 4.

CFBE41o- cells are homozygous for SNP I602S whereas CFTE29o- cells possess the wild type allele. The genomic DNA from both cell lines were subjected to PCR amplification followed by sequencing and RFLP analyses to determine the allele and genotypes. (A), CFTE29o- cell line possesses the wild type ′A′ allele (shown in box using the reverse primer sequence chromatogram), whereas the CFBE41o- cell line has a mutant ′C′ allele (shown similarly in box using the reverse primer sequence chromatogram) (B). The PCR products were also subjected to RFLP analyses to confirm the genotype of this 1805T/G SNP based on the allele specific digestion and distinct pattern on the agarose gel electrophoresis. (C), 16HBE14o- and CFTE29o- cell lines are homozygous wild type (AA or TT, lane 1, 3 digested with Pst1 and lane 2, 4 digested with Alu1 restriction enzymes) and CFBE41o- cell line is homozygous mutant (CC or GG, lane 5 digested with Pst1 and lane 6 digested with Alu1 restriction enzymes), the lane 7 loaded with low molecular weight ladder (NEB N3233S) to facilitate the band identification.