Fig 3. NaHS decreased the TGF-β1-stimulated overexpression of TGF-β receptor type II (TβR II) and TGF-β receptor type I (TβR I).

Pretreatment by the several concentrations of NaHS is given to HK-2 cells for 12 hours and then TGF-β1 was given to the cells for the following 36 hours. (A) NaHS decreased both TβR I and TβR II overexpression stimulated by TGF-β1 in a concentration-dependent manner in HK-2 cells. (B) Graphical representation of the relative quantification for TβR I and TβR II. The relative values were calculated by the density of TβR I and TβR II vs GAPDH (%). The values of mean ± SEM (n = 3) were gained from relative abundance quantified by densitometry and normalized to GAPDH. ^#P<0.05 vs. control group, *P<0.05 vs TGF-β1 group. One-way ANOVA followed by Tukey’s multiple comparison test.