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. 2016 Jan 13;11(1):e0147018. doi: 10.1371/journal.pone.0147018

Fig 9. NaHS inhibits the migration of HK-2 cells induced by TGF-β1.

Fig 9

(A,B,C,D) HK-2 cells were pretreated with or without NaHS (100μM) for 12 hours and then treated by TGF-β1 for 36 hours. Cells were detached and seeded to the upper section of the chamber, and then incubated for 24 h in a 5% CO2 incubator at 37°C. Purple color stands for cells that had gone through the membrane. (E) Graphical representation of the invaded cells. The values of mean ± SEM (n = 3) were gained from three randomly microscope fields in each chamber and normalized to control group. #P<0.05 vs. control group, *P0.05 vs TGF-β1 group. One-way ANOVA followed by Tukey’s multiple comparison test.