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. 2016 Jan 6;12(1):e1005370. doi: 10.1371/journal.ppat.1005370

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© 2016 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) PbUIS2 phosphatase domain (PD) dephosphorylated eIF2α-P in vitro. PfeIF2α-P[γ-³²P] was incubated with GST-PbUIS2PD in the presence of 5mM MnCl₂. (B) Characterization of PbUIS2 as a PP2C/PPM phosphatase. PbUIS2PD phosphatase activity was measured by malachite green colorimetric assay in the presence of EDTA, okadaic acid (OA), or CdCl₂. E: GST-PbUIS2PD; S: eIF2α-P. The inorganic phosphate was detected with malachite green by measuring the absorbance at 630 nm. Each value is the mean ± SD of three independent experiments.