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. 2016 Jan 7;11(1):e0145945. doi: 10.1371/journal.pone.0145945

Fig 5. Media osmolarity affects the binding of human anti-Vi and anti-O:9 antibodies to wild-type S. Typhi.

Fig 5

Five wild-type S. Typhi isolates, one from each geographical site to ensure the greatest geographical variation among strains studied, were cultured in LB broth containing 9, 85 or 500mM NaCl and incubated with either purified human anti-Vi (A) or anti-O:9 (B) antibodies or PBS. Levels of antibody binding to S. Typhi were assessed by flow cytometry. Values are mean of three separate experiments. Bars represent mean +/- SEM.