Roche 454 GS FLX Titanium+ |
Fragment, Mate Pair/ emPCR |
Pyrosequencing |
99.997% |
~700, maximum 1000 |
~1,000,000 |
23 h |
Long read length |
High reagent cost, homopolymer repeat errors |
De novo assemblies, metagenomics |
http://454.com/products/gs-flx-system/index.asp
|
Illumina- Solexa MiSeq |
Fragment, Mate Pair/ Solid phase |
Reversible terminator |
98% |
2 × 300 |
~25,000,000 |
~55 h 300 bp reads |
Widely used platform |
Short read length |
Small genomes, 16s amplicon, improving coverage |
http://systems.illumina.com/systems/miseq.html
|
Life Technologies SOLiD 5500 Series |
Fragment, Mate Pair/ emPCR |
Sequencing by ligation (Cleavable probe) |
99.99% |
Mate-paired 2 × 60, Paired-end 75 × 35 Fragment 75 |
1.2–1.4 billion |
1–2 weeks |
Low-cost |
Slow, issues with palindromic sequences reported |
Whole genome re-sequencing, variant analysis |
http://www.lifetechnologies.com/ (Huang et al. 2012) |
Life Technologies Ion Torrent |
Fragment, Mate Pair/ emPCR |
Sequential ion detection |
98% |
35–400 |
80,000,000 |
90 min |
Fast and inexpensive |
Reported homopolymer errors |
Small genomes, gene expression, ChiP-SEQ |
http://www.lifetechnologies.com/
|
Pacific Biosciences SMRT RS II |
Fragment only/ Single molecule |
Real-time |
99.999% |
N50 14,000, maximum >40,000 |
~50,000 |
30 min–4 h |
Longest read length, detects base modifications, fast |
Low single sequence accuracy 87% |
De novo assemblies, Base modification detection, Transcriptome sequencing |
(Murray et al. 2012; Chin et al. 2013) http://www.pacificbiosciences.com/
|