Fig 5.

Co-bombardment of HvVPS24-mEosFP with HvVPS60a-mCh induces re-localization of both proteins to large agglomerations in aleurone and subaleurone. a, b HvVPS24m-Ch and HvVPS60a-mCh are re-localized from cytoplasm to large globulare structures (arrowheads) in aleurone and subaleurone. Note the additional localization of VPS24m-Ch and VPS60a-mCh at the PM in aleurone (arrows). c, d 8 and 4 co-transformed cells within 5 and 1 biological replicates, respectively, were analysed in aleurone and subaleurone. Relative number of cells showing localization in cytosol (-c), at agglomerations (-a) and at the plasma membrane (-pm) was scored and compared with single transformation. Plus ends of the standard deviation are indicated. Confocal single scans were made 24 h after bombardment. Scale = 5 μm.