Figure 1.

S. flavescens extract promotes axonal extension on the CSPG coating. Cortical neurons were cultured for 1 day and then treated with S. flavescens extract (1 and 10 μg/ml) or vehicle solution. Four days after treatment, the cells were fixed and immunostained for pNF-H and MAP2. (A) Representative immunofluorescence images of pNF-H for each treatment. (B) The densities of pNF-H-positive axons per neuron were quantified for each treatment. The numbers in parentheses indicate the measured number of captured images. ^*p < 0.05 vs. CSPG coating/vehicle-treated group, One-way ANOVA post hoc Bonferroni test. Scale bar = 200 μm.